Fluorescent Modification of Adenosine 3′,5′-Monophosphate: Spectroscopic Properties and Activity in Enzyme Systems

The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N$^{6}$-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime,...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1972-07, Vol.177 (4045), p.279-280
Main Authors: Secrist, John A., Barrio, Jorge R., Leonard, Nelson J., Villar-Palasi, Carlos, Gilman, Alfred G.
Format: Article
Language:English
Subjects:
Acetaldehyde
Acetals
Adenosine Triphosphate
Agglutinins
Animals
Barrios
Biochemistry
Butchering
Cattle
Chemical Phenomena
Chemistry
Chlorine
Chromatography, Thin Layer
Cyclic AMP - chemical synthesis
Enzyme Activation
Enzymes
Fluorescence
Glycogen
Histones
Imidazoles - chemical synthesis
Muscles - enzymology
Myocardium - enzymology
Phosphoric Diester Hydrolases
Phosphorus Isotopes
Phosphotransferases
Protein Binding
Proteins
Quaternary ammonium compounds
Rabbits
Sodium
Spectrophotometry
Structure-Activity Relationship
Tritium
Ultraviolet Rays
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Summary:The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N$^{6}$-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.177.4045.279