Aeromonas salmonicida Growth in Response to Atlantic Salmon Mucins Differs between Epithelial Sites, Is Governed by Sialylated and N -Acetylhexosamine-Containing O -Glycans, and Is Affected by Ca 2

causes furunculosis in salmonids and is a threat to Atlantic salmon aquaculture. The epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. By using mass spectrometry to identify mucin glycan structures with and without enzymatic remo...

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Bibliographic Details
Published in:Infection and immunity 2017-08, Vol.85 (8)
Main Authors: Padra, János Tamás, Sundh, Henrik, Sundell, Kristina, Venkatakrishnan, Vignesh, Jin, Chunsheng, Samuelsson, Tore, Karlsson, Niclas G, Lindén, Sara K
Format: Article
Language:English
Subjects:
Acetylglucosamine - metabolism
Aeromonas salmonicida - growth & development
Aeromonas salmonicida - pathogenicity
Aeromonas salmonicida - physiology
Animals
Aquaculture
Calcium - metabolism
Furunculosis - microbiology
Glycosylation
Hexosamines - chemistry
Intestines - chemistry
Mass Spectrometry
Mucins - chemistry
Mucins - metabolism
Polysaccharides - chemistry
Polysaccharides - metabolism
Salmo salar - metabolism
Sialic Acids - metabolism
Skin - chemistry
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Summary:causes furunculosis in salmonids and is a threat to Atlantic salmon aquaculture. The epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. By using mass spectrometry to identify mucin glycan structures with and without enzymatic removal of glycan residues, coupled to measurements of bacterial growth, we show here that the complex Atlantic salmon intestinal mucin glycans enhance growth, whereas the more simple skin mucin glycans do not. Of the glycan residues present terminally on the salmon mucins, only -acetylglucosamine (GlcNAc) enhances growth. Sialic acids, which have an abundance of 75% among terminal glycans from skin and of
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00189-17