Responses of Cattle to Gastrointestinal Colonization by Escherichia coli O157:H7

• Published in: Infection and Immunity 2008-11, Vol.76 (11), p.5366-5372
• Main Authors: Nart, Pablo, Naylor, Stuart W, Huntley, John F, McKendrick, Iain J, Gally, David L, Low, J. Christopher
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - immunology; Antigens, Bacterial - immunology; Bacterial Infections; Bacteriology; Biological and medical sciences; Cattle; Escherichia coli; Escherichia coli Infections - immunology; Escherichia coli Infections - pathology; Escherichia coli Infections - veterinary; Escherichia coli O157 - immunology; Fundamental and applied biological sciences. Psychology; Immunity, Mucosal; Intestinal Mucosa - immunology; Intestinal Mucosa - microbiology; Intestinal Mucosa - pathology; Microbiology; Microscopy, Electron; Miscellaneous; Rectum - immunology; Rectum - microbiology; Rectum - pathology
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.01223-07

Recent research has established that the terminal rectum is the predominant colonization site of enterohemorrhagic Escherichia coli O157:H7 in cattle. The main aim of the present work was to investigate pathological changes and associated immune responses at this site in animals colonized with E. coli O157:H7. Tissue and gastrointestinal samples from a total of 22 weaned Holstein-cross calves challenged with E. coli O157:H7 were analyzed for bacterial colonization and pathology. Five unexposed age-matched calves were used as comparative negative controls. E. coli O157:H7 bacteria induced histopathological alterations of the rectal mucosa with enterocyte remodeling. This was often associated with removal of the colonized epithelial layer. Immunogold labeling and transmission electron microscopy (TEM) showed E. coli O157 bacteria on pedestals, as part of attaching and effacing lesions. These pathological changes induced a local infiltration of neutrophils that was quantified as larger in infected animals. Rectal mucosal immunoglobulin A responses were detected against the E. coli O157:H7 antigen. This work presents evidence that E. coli O157:H7 is not a commensal bacteria in the bovine host and that the mucosal damage produced by E. coli O157:H7 colonization of the terminal rectum induces a quantifiable innate immune response and production of specific mucosal antibodies.