Loading…

Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera

The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent ass...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 2008-01, Vol.46 (1), p.56-61
Main Authors: Fujima, Akikazu, Ochiai, Yoshitsugu, Saito, Aya, Omori, Yuki, Noda, Atsuya, Kazuyama, Yukumasa, Shoji, Hiroshi, Tanabayashi, Kiyoshi, Ueda, Fukiko, Yoshikawa, Yasuhiro, Hondo, Ryo
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23
cites cdi_FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23
container_end_page 61
container_issue 1
container_start_page 56
container_title Journal of Clinical Microbiology
container_volume 46
creator Fujima, Akikazu
Ochiai, Yoshitsugu
Saito, Aya
Omori, Yuki
Noda, Atsuya
Kazuyama, Yukumasa
Shoji, Hiroshi
Tanabayashi, Kiyoshi
Ueda, Fukiko
Yoshikawa, Yasuhiro
Hondo, Ryo
description The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with recombinant herpes B virus glycoprotein D (gD) and HSV-1 and HSV-2 gG (gG-1 and gG-2, respectively) to discriminate between the three primate herpesvirus infections. The secreted form of gD, gDdTM, was used to detect antibody to herpes B virus gD. Sera positive for herpes B virus, HSV-1, and HSV-2 showed specific reactions to gD, gG-1, and gG-2, respectively. Sera collected from humans and rhesus macaques were investigated for the presence of antibodies to the recombinant proteins of the three herpesviruses. The results suggested that the approach is able to discriminate between herpes B virus and HSV infections. The ELISA was also found to be able to detect infections with multiple primate herpesviruses and may have the potential to identify a subsequent infection in individuals that have already been infected with another herpesvirus. In addition, we found evidence of a greater cross-reactivity of herpes B virus with HSV-1 than with HSV-2. It is suggested that the ELISA with the recombinant antigens is useful not only for the serodiagnosis of primate herpesvirus infections but also for elucidation of the seroprevalence of herpesviruses in humans and primates.
doi_str_mv 10.1128/JCM.00342-07
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1128_JCM_00342_07</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20565944</sourcerecordid><originalsourceid>FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxSMEokvhxhl8gRMpYyeOnUulsnwsqBWHbRE3a-K1d10l8WInwPLX4-1GLUhIXMaamZ-e3vhl2VMKJ5Qy-frT_OIEoChZDuJeNqNQy7yq4Ov9bAZQ85zSQhxlj2K8BqBlyfnD7IiKWtYMYJb9euuiDq5zPQ7O98RbctYPrvGrHRk8WZiwNZG8IV9cGCOxwXf_2i9dt23Nz4m63O1nlGC_Ioy4nizGDvub9gI1fhsNWZqAj7MHFttonkzvcXb1_t3lfJGff_7wcX52nmtelENOheFNs0LOmZScUm4ry2orta5SZRILLm2NfKWpbHiDUGhhTWO5FMxIw4rj7PSgux2bzqy06YeArdqmqzHslEen_t70bqPW_rtijJWM10ng5SQQfDIfB9WlTzNti73xY1QCqARB_w8y4BWvyzKBrw6gDj7GYOytGwpqn6pKqaqbVBWIhD_784I7eIoxAS8mAKPG1gbstYu3HAMGdQ17g88P3MatNz9cMApjp651p8pKUcWrO8KiV7gOSeVqyYAWALJM7kXxGxIwvqg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20565944</pqid></control><display><type>article</type><title>Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera</title><source>American Society for Microbiology</source><source>PubMed Central</source><creator>Fujima, Akikazu ; Ochiai, Yoshitsugu ; Saito, Aya ; Omori, Yuki ; Noda, Atsuya ; Kazuyama, Yukumasa ; Shoji, Hiroshi ; Tanabayashi, Kiyoshi ; Ueda, Fukiko ; Yoshikawa, Yasuhiro ; Hondo, Ryo</creator><creatorcontrib>Fujima, Akikazu ; Ochiai, Yoshitsugu ; Saito, Aya ; Omori, Yuki ; Noda, Atsuya ; Kazuyama, Yukumasa ; Shoji, Hiroshi ; Tanabayashi, Kiyoshi ; Ueda, Fukiko ; Yoshikawa, Yasuhiro ; Hondo, Ryo</creatorcontrib><description>The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with recombinant herpes B virus glycoprotein D (gD) and HSV-1 and HSV-2 gG (gG-1 and gG-2, respectively) to discriminate between the three primate herpesvirus infections. The secreted form of gD, gDdTM, was used to detect antibody to herpes B virus gD. Sera positive for herpes B virus, HSV-1, and HSV-2 showed specific reactions to gD, gG-1, and gG-2, respectively. Sera collected from humans and rhesus macaques were investigated for the presence of antibodies to the recombinant proteins of the three herpesviruses. The results suggested that the approach is able to discriminate between herpes B virus and HSV infections. The ELISA was also found to be able to detect infections with multiple primate herpesviruses and may have the potential to identify a subsequent infection in individuals that have already been infected with another herpesvirus. In addition, we found evidence of a greater cross-reactivity of herpes B virus with HSV-1 than with HSV-2. It is suggested that the ELISA with the recombinant antigens is useful not only for the serodiagnosis of primate herpesvirus infections but also for elucidation of the seroprevalence of herpesviruses in humans and primates.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.00342-07</identifier><identifier>PMID: 17989200</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Antibodies, Viral - blood ; Antigens, Viral - genetics ; Biological and medical sciences ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay - methods ; Fundamental and applied biological sciences. Psychology ; Herpes simplex virus 1 ; Herpes simplex virus 2 ; Herpesviridae Infections - diagnosis ; Herpesviridae Infections - immunology ; Herpesvirus ; Herpesvirus 1, Cercopithecine - immunology ; Herpesvirus 1, Human - immunology ; Herpesvirus 2, Human - immunology ; Human viral diseases ; Humans ; Infectious diseases ; Macaca mulatta ; Medical sciences ; Microbiology ; Miscellaneous ; Recombinant Proteins - genetics ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye ; Viral Envelope Proteins ; Virology</subject><ispartof>Journal of Clinical Microbiology, 2008-01, Vol.46 (1), p.56-61</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23</citedby><cites>FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224259/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224259/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=20209909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17989200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujima, Akikazu</creatorcontrib><creatorcontrib>Ochiai, Yoshitsugu</creatorcontrib><creatorcontrib>Saito, Aya</creatorcontrib><creatorcontrib>Omori, Yuki</creatorcontrib><creatorcontrib>Noda, Atsuya</creatorcontrib><creatorcontrib>Kazuyama, Yukumasa</creatorcontrib><creatorcontrib>Shoji, Hiroshi</creatorcontrib><creatorcontrib>Tanabayashi, Kiyoshi</creatorcontrib><creatorcontrib>Ueda, Fukiko</creatorcontrib><creatorcontrib>Yoshikawa, Yasuhiro</creatorcontrib><creatorcontrib>Hondo, Ryo</creatorcontrib><title>Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with recombinant herpes B virus glycoprotein D (gD) and HSV-1 and HSV-2 gG (gG-1 and gG-2, respectively) to discriminate between the three primate herpesvirus infections. The secreted form of gD, gDdTM, was used to detect antibody to herpes B virus gD. Sera positive for herpes B virus, HSV-1, and HSV-2 showed specific reactions to gD, gG-1, and gG-2, respectively. Sera collected from humans and rhesus macaques were investigated for the presence of antibodies to the recombinant proteins of the three herpesviruses. The results suggested that the approach is able to discriminate between herpes B virus and HSV infections. The ELISA was also found to be able to detect infections with multiple primate herpesviruses and may have the potential to identify a subsequent infection in individuals that have already been infected with another herpesvirus. In addition, we found evidence of a greater cross-reactivity of herpes B virus with HSV-1 than with HSV-2. It is suggested that the ELISA with the recombinant antigens is useful not only for the serodiagnosis of primate herpesvirus infections but also for elucidation of the seroprevalence of herpesviruses in humans and primates.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens, Viral - genetics</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpes simplex virus 1</subject><subject>Herpes simplex virus 2</subject><subject>Herpesviridae Infections - diagnosis</subject><subject>Herpesviridae Infections - immunology</subject><subject>Herpesvirus</subject><subject>Herpesvirus 1, Cercopithecine - immunology</subject><subject>Herpesvirus 1, Human - immunology</subject><subject>Herpesvirus 2, Human - immunology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Macaca mulatta</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Recombinant Proteins - genetics</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><subject>Viral Envelope Proteins</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkc1v1DAQxSMEokvhxhl8gRMpYyeOnUulsnwsqBWHbRE3a-K1d10l8WInwPLX4-1GLUhIXMaamZ-e3vhl2VMKJ5Qy-frT_OIEoChZDuJeNqNQy7yq4Ov9bAZQ85zSQhxlj2K8BqBlyfnD7IiKWtYMYJb9euuiDq5zPQ7O98RbctYPrvGrHRk8WZiwNZG8IV9cGCOxwXf_2i9dt23Nz4m63O1nlGC_Ioy4nizGDvub9gI1fhsNWZqAj7MHFttonkzvcXb1_t3lfJGff_7wcX52nmtelENOheFNs0LOmZScUm4ry2orta5SZRILLm2NfKWpbHiDUGhhTWO5FMxIw4rj7PSgux2bzqy06YeArdqmqzHslEen_t70bqPW_rtijJWM10ng5SQQfDIfB9WlTzNti73xY1QCqARB_w8y4BWvyzKBrw6gDj7GYOytGwpqn6pKqaqbVBWIhD_784I7eIoxAS8mAKPG1gbstYu3HAMGdQ17g88P3MatNz9cMApjp651p8pKUcWrO8KiV7gOSeVqyYAWALJM7kXxGxIwvqg</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Fujima, Akikazu</creator><creator>Ochiai, Yoshitsugu</creator><creator>Saito, Aya</creator><creator>Omori, Yuki</creator><creator>Noda, Atsuya</creator><creator>Kazuyama, Yukumasa</creator><creator>Shoji, Hiroshi</creator><creator>Tanabayashi, Kiyoshi</creator><creator>Ueda, Fukiko</creator><creator>Yoshikawa, Yasuhiro</creator><creator>Hondo, Ryo</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080101</creationdate><title>Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera</title><author>Fujima, Akikazu ; Ochiai, Yoshitsugu ; Saito, Aya ; Omori, Yuki ; Noda, Atsuya ; Kazuyama, Yukumasa ; Shoji, Hiroshi ; Tanabayashi, Kiyoshi ; Ueda, Fukiko ; Yoshikawa, Yasuhiro ; Hondo, Ryo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens, Viral - genetics</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpes simplex virus 1</topic><topic>Herpes simplex virus 2</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesviridae Infections - immunology</topic><topic>Herpesvirus</topic><topic>Herpesvirus 1, Cercopithecine - immunology</topic><topic>Herpesvirus 1, Human - immunology</topic><topic>Herpesvirus 2, Human - immunology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Macaca mulatta</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Recombinant Proteins - genetics</topic><topic>Viral diseases</topic><topic>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</topic><topic>Viral Envelope Proteins</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujima, Akikazu</creatorcontrib><creatorcontrib>Ochiai, Yoshitsugu</creatorcontrib><creatorcontrib>Saito, Aya</creatorcontrib><creatorcontrib>Omori, Yuki</creatorcontrib><creatorcontrib>Noda, Atsuya</creatorcontrib><creatorcontrib>Kazuyama, Yukumasa</creatorcontrib><creatorcontrib>Shoji, Hiroshi</creatorcontrib><creatorcontrib>Tanabayashi, Kiyoshi</creatorcontrib><creatorcontrib>Ueda, Fukiko</creatorcontrib><creatorcontrib>Yoshikawa, Yasuhiro</creatorcontrib><creatorcontrib>Hondo, Ryo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujima, Akikazu</au><au>Ochiai, Yoshitsugu</au><au>Saito, Aya</au><au>Omori, Yuki</au><au>Noda, Atsuya</au><au>Kazuyama, Yukumasa</au><au>Shoji, Hiroshi</au><au>Tanabayashi, Kiyoshi</au><au>Ueda, Fukiko</au><au>Yoshikawa, Yasuhiro</au><au>Hondo, Ryo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>46</volume><issue>1</issue><spage>56</spage><epage>61</epage><pages>56-61</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with recombinant herpes B virus glycoprotein D (gD) and HSV-1 and HSV-2 gG (gG-1 and gG-2, respectively) to discriminate between the three primate herpesvirus infections. The secreted form of gD, gDdTM, was used to detect antibody to herpes B virus gD. Sera positive for herpes B virus, HSV-1, and HSV-2 showed specific reactions to gD, gG-1, and gG-2, respectively. Sera collected from humans and rhesus macaques were investigated for the presence of antibodies to the recombinant proteins of the three herpesviruses. The results suggested that the approach is able to discriminate between herpes B virus and HSV infections. The ELISA was also found to be able to detect infections with multiple primate herpesviruses and may have the potential to identify a subsequent infection in individuals that have already been infected with another herpesvirus. In addition, we found evidence of a greater cross-reactivity of herpes B virus with HSV-1 than with HSV-2. It is suggested that the ELISA with the recombinant antigens is useful not only for the serodiagnosis of primate herpesvirus infections but also for elucidation of the seroprevalence of herpesviruses in humans and primates.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>17989200</pmid><doi>10.1128/JCM.00342-07</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0095-1137
ispartof Journal of Clinical Microbiology, 2008-01, Vol.46 (1), p.56-61
issn 0095-1137
1098-660X
1098-5530
language eng
recordid cdi_crossref_primary_10_1128_JCM_00342_07
source American Society for Microbiology; PubMed Central
subjects Animals
Antibodies, Viral - blood
Antigens, Viral - genetics
Biological and medical sciences
Cross Reactions
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Herpes simplex virus 1
Herpes simplex virus 2
Herpesviridae Infections - diagnosis
Herpesviridae Infections - immunology
Herpesvirus
Herpesvirus 1, Cercopithecine - immunology
Herpesvirus 1, Human - immunology
Herpesvirus 2, Human - immunology
Human viral diseases
Humans
Infectious diseases
Macaca mulatta
Medical sciences
Microbiology
Miscellaneous
Recombinant Proteins - genetics
Viral diseases
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
Viral Envelope Proteins
Virology
title Discrimination of Antibody to Herpes B Virus from Antibody to Herpes Simplex Virus Types 1 and 2 in Human and Macaque Sera
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A13%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Discrimination%20of%20Antibody%20to%20Herpes%20B%20Virus%20from%20Antibody%20to%20Herpes%20Simplex%20Virus%20Types%201%20and%202%20in%20Human%20and%20Macaque%20Sera&rft.jtitle=Journal%20of%20Clinical%20Microbiology&rft.au=Fujima,%20Akikazu&rft.date=2008-01-01&rft.volume=46&rft.issue=1&rft.spage=56&rft.epage=61&rft.pages=56-61&rft.issn=0095-1137&rft.eissn=1098-660X&rft.coden=JCMIDW&rft_id=info:doi/10.1128/JCM.00342-07&rft_dat=%3Cproquest_cross%3E20565944%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c534t-17e5bbda552885115f6f29f8cc69f828a358f9a5dc18b5ba03c7febf5872e8e23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20565944&rft_id=info:pmid/17989200&rfr_iscdi=true