Evaluation of a Loop-Mediated Isothermal Amplification Method Using Fecal Specimens for Differential Detection of Taenia Species from Humans

We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the mul...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-09, Vol.48 (9), p.3350-3352
Main Authors: Nkouawa, Agathe, Sako, Yasuhito, Li, Tiaoying, Chen, Xingwang, Wandra, Toni, Swastika, I. Kadek, Nakao, Minoru, Yanagida, Tetsuya, Nakaya, Kazuhiro, Qiu, Dongchuan, Ito, Akira
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Feces - parasitology
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Nucleic Acid Amplification Techniques - methods
Parasitology
Parasitology - methods
Sensitivity and Specificity
Taenia - classification
Taenia - genetics
Taenia - isolation & purification
Taeniasis - diagnosis
Taeniasis - parasitology
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Summary:We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00697-10