Clostridium difficile Strains from Community-Associated Infections

Clostridium difficile isolates from presumed community-associated infections (n = 92) were characterized by toxinotyping, pulsed-field gel electrophoresis, tcdC and cdtB PCR, and antimicrobial susceptibility. Nine toxinotypes (TOX) and 31 PFGE patterns were identified. TOX 0 (48, 52%), TOX III (18,...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-09, Vol.47 (9), p.3004-3007
Main Authors: Limbago, Brandi M, Long, Cherie M, Thompson, Angela D, Killgore, George E, Hannett, George E, Havill, Nancy L, Mickelson, Stephanie, Lathrop, Sarah, Jones, Timothy F, Park, Mahin M, Harriman, Kathleen H, Gould, L. Hannah, McDonald, L. Clifford, Angulo, Frederick J
Format: Article
Language:English
Subjects:
ADP Ribose Transferases - genetics
Bacterial Proteins - genetics
Bacterial Toxins - analysis
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Clostridium difficile
Clostridium difficile - classification
Clostridium difficile - isolation & purification
Cluster Analysis
Community-Acquired Infections - microbiology
DNA Fingerprinting
Electrophoresis, Gel, Pulsed-Field
Enterocolitis, Pseudomembranous - microbiology
Epidemiology
Fundamental and applied biological sciences. Psychology
Genotype
Humans
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Molecular Epidemiology
Polymerase Chain Reaction - methods
Repressor Proteins - genetics
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Summary:Clostridium difficile isolates from presumed community-associated infections (n = 92) were characterized by toxinotyping, pulsed-field gel electrophoresis, tcdC and cdtB PCR, and antimicrobial susceptibility. Nine toxinotypes (TOX) and 31 PFGE patterns were identified. TOX 0 (48, 52%), TOX III (18, 20%), and TOX V (9, 10%) were the most common; three isolates were nontoxigenic.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00964-09