Mycobacterium tuberculosis in Ontario, Canada: Insights from IS 6110 Restriction Fragment Length Polymorphism and Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Genotyping

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS 6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were nece...

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Bibliographic Details
Published in:Journal of clinical microbiology 2009-08, Vol.47 (8), p.2651-2654
Main Authors: Alexander, David C., Guthrie, Jennifer L., Pyskir, Daria, Maki, Anne, Kurepina, Natalia, Kreiswirth, Barry N., Chedore, Pamela, Drews, Steven J., Jamieson, Frances
Format: Article
Language:English
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Summary:A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS 6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01946-08