A Yersinia T6SS Effector YezP Engages the Hemin Uptake Receptor HmuR and ZnuABC for Zn 2+ Acquisition

• Published in: Applied and environmental microbiology 2023-07, Vol.89 (7), p.e0024023
• Main Authors: Liu, Hai, Wei, Zhiyan, Li, Jiali, Liu, Xingyu, Zhu, Lingfang, Wang, Yao, Wang, Tietao, Li, Changfu, Shen, Xihui
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological Transport; Hemin - metabolism; Humans; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Yersinia - metabolism; Yersinia pseudotuberculosis Infections; Zinc - metabolism
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.00240-23

Metal ions are essential nutrients for all life forms, and restriction of metal ion availability is an effective host defense against bacterial infection. Meanwhile, bacterial pathogens have developed equally effective means to secure their metal ion supply. The enteric pathogen Yersinia pseudotuberculosis was found to uptake zinc using the T6SS4 effector YezP, which is essential for Zn acquisition and bacterial survival under oxidative stress. However, the mechanism of this zinc uptake pathway has not been fully elucidated. Here, we identified the hemin uptake receptor HmuR for YezP, which can mediate import of Zn into the periplasm by the YezP-Zn complex and demonstrated that YezP functions extracellularly. This study also confirmed that the ZnuCB transporter is the inner membrane transporter for Zn from the periplasm to cytoplasm. Overall, our results reveal the complete T6SS/YezP/HmuR/ZnuABC pathway, wherein multiple systems are coupled to support zinc uptake by Y. pseudotuberculosis under oxidative stress. Identifying the transporters involved in import of metal ions under normal physiological growth conditions in bacterial pathogens will clarify its pathogenic mechanism. Y. pseudotuberculosis YPIII, a common foodborne pathogen that infects animals and humans, uptake zinc via the T6SS4 effector YezP. However, the outer and inner transports involved in Zn acquisition remain unknown. The important outcomes of this study are the identification of the hemin uptake receptor HmuR and inner membrane transporter ZnuCB that import Zn into the cytoplasm via the YezP-Zn complex, and elucidation of the complete Zn acquisition pathway consisting of T6SS, HmuRSTUV, and ZnuABC, thereby providing a comprehensive view of T6SS-mediated ion transport and its functions.