2D [¹H,¹³C] NMR study of carbon fluxes during glucose utilization by Escherichia coli MG1655

Carbon fluxes through main pathways of glucose utilization in Escherichia coli cells-glycolysis, pentose phosphate pathway (PPP), and Enther-Doudoroff pathway (EDP)--were studied. Their ratios were analyzed in E. coli strains MG1655, MG1655Δ(edd-eda), MG1655Δ(zwf, edd-eda), and MG1655Δ(pgi, edd-eda)...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2008-03, Vol.44 (2), p.151-157
Main Authors: Kivero, A. D, Bocharov, E. V, Doroshenko, V. G, Sobol, A. G, Dubinnyi, M. A, Arseniev, A. S
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Life Sciences
Medical Microbiology
Microbiology
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Summary:Carbon fluxes through main pathways of glucose utilization in Escherichia coli cells-glycolysis, pentose phosphate pathway (PPP), and Enther-Doudoroff pathway (EDP)--were studied. Their ratios were analyzed in E. coli strains MG1655, MG1655Δ(edd-eda), MG1655Δ(zwf, edd-eda), and MG1655Δ(pgi, edd-eda). It was shown that the carbon flux through glycolysis was the main route of glucose utilization, averaging ca. 80%. Inactivation of EDP did not affect growth parameters. Nevertheless, it altered carbon fluxes through the tricarboxylic acid cycles and energy metabolism in the cell. Inactivation of PPP decreased growth rate to a lesser degree than glycolysis inactivation.
ISSN:0003-6838
1608-3024
DOI:10.1134/S000368380802004X