Evaluation of the relative cell surface charge by using microbial adhesion to hydrocarbon

A simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference between these two affinities can provide the rela...

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Bibliographic Details
Published in:Microbiology (New York) 2011-08, Vol.80 (4), p.488-491
Main Authors: Hamadi, Fatima, Latrache, Hassan, Zahir, Hafida, Bengourram, Jamaa, Kouider, Nourreeddine, Elghmari, Abderrahmene, Habbari, Khalid
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
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Summary:A simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference between these two affinities can provide the relative cell surface charge. The application of this method for Staphylococcus aureus and Escherichia coli show that the profile of surface charge evolution as a function of pH was similar to these obtained by microelectrophoresis method.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261711040072