Anoxygenic phototrophic bacteria from microbial communities of Goryachinsk thermal spring (Baikal Area, Russia)

• Published in: Microbiology (New York) 2014-07, Vol.83 (4), p.407-421
• Main Authors: Kalashnikov, A. M., Gaisin, V. A., Sukhacheva, M. V., Namsaraev, B. B., Panteleeva, A. N., Nuyanzina-Boldareva, E. N., Kuznetsov, B. B., Gorlenko, V. M.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261714040080

Species composition of anoxygenic phototrophic bacteria in microbial mats of the Goryachinsk thermal spring was investigated along the temperature gradient. The spring belonging to nitrogenous alkaline hydrotherms is located at the shore of Lake Baikal 188 km north-east from Ulan-Ude. The water is of the sulfate-sodium type, contains trace amounts of sulfide, and salinity does not exceed 0.64 g/L, pH 9.5. The temperature at the outlet of the spring may reach 54°C. The cultures of filamentous anoxygenic phototrophic bacteria, nonsulfur and sulfur purple bacteria, and aerobic anoxygenic phototrophic bacteria were identified using the puf LM molecular marker. The fmo A marker was used for identification of green sulfur bacteria. Filamentous cyanobacteria predominated in the mats, with anoxygenic phototrophs comprising a minor component of the phototrophic communities. Thermophilic bacteria Chloroflexus aurantiacus were detected in the samples from both the thermophilic and mesophilic mats. Cultures of nonsulfur purple bacteria similar to Blastochloris sulfoviridis and Rhodomicrobium vannielii were isolated from the mats developed at high (50.6–49.4°C) and low temperatures (45–20°C). Purple sulfur bacteria Allochromatium sp. and Thiocapsa sp., as well as green sulfur bacteria Chlorobium sp., were revealed in low-temperature mats. Truly thermophilic purple and green sulfur bacteria were not found in the spring. Anoxygenic phototrophic bacteria found in the spring were typical of the sulfur communities, for which the sulfur cycle is mandatory. The presence of aerobic bacteriochlorophyll a -containing bacteria identified as Agrobacterium ( Rhizobium ) tumifaciens in the mesophilic (20°C) mat is of interest.