Disruption of bacterial biofilms using recombinant dispersin B

A synthetic gene encoding dispersin B of Aggregatibacter actinomycetemcomitans was cloned and expressed in Escherichia coli cells. Procedure for purification of recombinant dispersin B was developed, and its in vitro activity was determined. The enzyme was used in experiments on disruption of the bi...

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Bibliographic Details
Published in:Microbiology (New York) 2015-07, Vol.84 (4), p.498-501
Main Authors: Dobrynina, O. Yu, Bolshakova, T. N, Umyarov, A. M, Boksha, I. S, Lavrova, N. V, Grishin, A. V, Lyashchuk, A. M, Galushkina, Z. M, Avetisian, L. R, Chernukha, M. Yu, Shaginian, I. A, Lunin, V. G, Karyagina, A. S
Format: Article
Language:English
Subjects:
Achromobacter xylosoxidans
Actinobacillus actinomycetemcomitans
biofilm
Biomedical and Life Sciences
Burkholderia cenocepacia
Escherichia coli
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
microorganisms
Staphylococcus epidermidis
synthetic genes
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Summary:A synthetic gene encoding dispersin B of Aggregatibacter actinomycetemcomitans was cloned and expressed in Escherichia coli cells. Procedure for purification of recombinant dispersin B was developed, and its in vitro activity was determined. The enzyme was used in experiments on disruption of the biofilms formed by various microorganisms. It exhibited high activity against Staphylococcus epidermidis biofilms. The biofilms formed by Burkholderia cenocepacia and Achromobacter xylosoxidans were more resistant to the recombinant enzyme.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261715040062