Simple colorimetric detection of doxycycline and oxytetracycline using unmodified gold nanoparticles

• Published in: Optics and spectroscopy 2014-08, Vol.117 (2), p.250-255
• Main Authors: Li, Jie, Fan, Shumin, Li, Zhigang, Xie, Yuanzhe, Wang, Rui, Ge, Baoyu, Wu, Jing, Wang, Ruiyong
• Format: Article
• Language: English
• Subjects: Condensed-Matter Spectroscopy; Lasers; Optical Devices; Optics; Photonics; Physics; Physics and Astronomy
• ISSN: 0030-400X; 1562-6911
• DOI: 10.1134/S0030400X14070212

The interaction between tetracycline antibiotics and gold nanoparticles was studied. With citrate-coated gold nanoparticles as colorimetric probe, a simple and rapid detection method for doxycycline and oxytetracycline has been developed. This method relies on the distance-dependent optical properties of gold nanoparticles. In weakly acidic buffer medium, doxycycline and oxytetracycline could rapidly induce the aggregation of gold nanoparticles, resulting in red-to-blue (or purple) colour change. The experimental parameters were optimized with regard to pH, the concentration of the gold nanoparticles and the reaction time. Under optimal experimental conditions, the linear range of the colorimetric sensor for doxycycline/oxytetracycline was 0.06–0.66 and 0.59–8.85 μg mL −1 , respectively. The corresponding limit of detection for doxycycline and oxytetracycline was 0.0086 and 0.0838 μg mL −1 , respectively. This assay was sensitive, selective, simple and readily used to detect tetracycline antibiotics in food products.