Blue native electrophoresis as a tool for detection of PIP-containing protein complexes in the plasma membranes

Blue native electrophoresis (BN-PAGE) is presently considered as one of effective methods for the identification of membrane protein complexes. The choice of a nonionic detergent and the detergent to protein ratio are critically important. Our experiments with plasma membranes of etiolated pea (Pisu...

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Bibliographic Details
Published in:Russian journal of plant physiology 2013, Vol.60 (4), p.563-570
Main Authors: Shevyreva, T. A, Piotrovskii, M. S, Belugin, B. V, Zhestkova, I. M, Trofimova, M. S
Format: Article
Language:English
Subjects:
aquaporins
Biomedical and Life Sciences
detergents
digitonin
electrophoresis
etiolation
Life Sciences
Methods
peas
Pisum sativum
Plant Physiology
Plant Sciences
seedlings
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Summary:Blue native electrophoresis (BN-PAGE) is presently considered as one of effective methods for the identification of membrane protein complexes. The choice of a nonionic detergent and the detergent to protein ratio are critically important. Our experiments with plasma membranes of etiolated pea (Pisum sativum L.) seedlings showed that various nonionic detergents—digitonin, dodecyl maltoside, and Triton X-100—solubilized similar assortments of protein complexes. Irrespective of the detergent type, PIP aquaporins were always observed in the 440-kD protein complex. Only in the case of dodecyl maltoside, the PIP aquaporins were also revealed in the complexes with the lower and higher molecular weights when the detergent/protein ratio increased.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443713040158