Current technics for visualizing RNA in a cell

The study of RNA functions in a cell is based on the methods of labeling and detection of these molecules. In recent years, considerable progress has been made in this area, making it possible to detect RNA in amounts to one molecule with high specificity and to track the dynamics of spatial RNA dis...

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Bibliographic Details
Published in:Russian journal of genetics 2017-10, Vol.53 (10), p.1080-1090
Main Authors: Ibragimov, A. N., Kozlov, E. N., Kurbidaeva, A. S., Ryabichko, S. S., Shidlovskii, Yu. V.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedicine
Human Genetics
Microbial Genetics and Genomics
Reviews and Theoretical Articles
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Summary:The study of RNA functions in a cell is based on the methods of labeling and detection of these molecules. In recent years, considerable progress has been made in this area, making it possible to detect RNA in amounts to one molecule with high specificity and to track the dynamics of spatial RNA distribution in the living cell. The review presents the latest developments in the field of in situ hybridization and the use of RNA aptamers and RNA-binding proteins. The prospects of using the CRISPR-Cas system for RNA visualization are discussed in detail.
ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795417100040