Some characteristics of transgenic clones of mouse R1 line embryonic stem cells

Transgenic clones of mouse embryonic stem cells of the R1 line were received by transfection of plasmid linear vectors. The changes in the transgene structure during its integration into the genome of the target cells were investigated. Displacements were found on the flanks of the integrated transg...

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Bibliographic Details
Published in:Biology bulletin of the Russian Academy of Sciences 2016-07, Vol.43 (4), p.283-289
Main Authors: Drozd, S. F., Surkov, S. A., Glazkov, M. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Biology
Ecology
Genetics
Life Sciences
Zoology
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Summary:Transgenic clones of mouse embryonic stem cells of the R1 line were received by transfection of plasmid linear vectors. The changes in the transgene structure during its integration into the genome of the target cells were investigated. Displacements were found on the flanks of the integrated transgene. It was found that multicopy tandem structures are formed in head–tail orientation at the transgene integration. It was noted that the number of copies of the integrated transgenes varies considerably, but the introduction of DNA fragments isolated from the nuclear envelopes into the flanks of the transgene normalizes the number of its copies.
ISSN:1062-3590
1608-3059
DOI:10.1134/S1062359016030031