Modifications in major satellite methylation in the nucleus of a two-cell mouse embryo with respect to developmental conditions

A study was conducted of the degree of DNA methylation in the nucleus, in particular, of the major satellite in two-cell mouse embryos developing in the maternal organism, in standard culture medium M16, used for cultivating mouse embryos; and M2 media used for manipulating embryos in air. Two-cell...

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Bibliographic Details
Published in:Russian journal of developmental biology 2009-09, Vol.40 (5), p.305-312
Main Authors: Kuznetsova, I. S, Noniashvili, E. M, Gavrilova, E. V, Dyban, A. P
Format: Article
Language:English
Subjects:
Animal Anatomy
Biomedical and Life Sciences
Developmental Biology
embryo
epigenomic reprogramming
Histology
Life Sciences
major satellite
methylation
Molecular Biology of Development
Morphology
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Summary:A study was conducted of the degree of DNA methylation in the nucleus, in particular, of the major satellite in two-cell mouse embryos developing in the maternal organism, in standard culture medium M16, used for cultivating mouse embryos; and M2 media used for manipulating embryos in air. Two-cell embryo nuclei at 44-46 h after injections of chorionic hormone were investigated. The results are evidence for the dependence of the major satellite's methylation level on the developmental conditions of embryos. The methylation level of the nuclear DNA was shown to increase with a deterioration of environmental conditions. It was reported that in the case of cultivation in M2 media unsuitable for long cultivation, the DNAís methylation level, the major satellite in particular, was higher compared to other embryo groups. Accordingly, not only a significant number of genes but also sequences of satellite DNA are involved in epigenetic regulation.
ISSN:1062-3604
1608-3326
DOI:10.1134/S1062360409050051