Generation of reactive oxygen species during pollen grain germination

The formation of reactive oxygen species in pollen at the early germination stage, which precedes the formation of the pollen tube, was studied. During this period, pollen grain is being hydrated, abruptly increasing its volume, and it passes from the resting state to active metabolism. Fluorescent...

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Bibliographic Details
Published in:Russian journal of developmental biology 2009-11, Vol.40 (6), p.345-353
Main Authors: Smirnova, A. V, Matveyeva, N. P, Polesskaya, O. G, Yermakov, I. P
Format: Article
Language:English
Subjects:
Animal Anatomy
Biology of Plant Development
Biomedical and Life Sciences
DCFH-DA and MitoSOX dyes
Developmental Biology
germination
Histology
Life Sciences
Morphology
pollen grain
reactive oxygen species
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Summary:The formation of reactive oxygen species in pollen at the early germination stage, which precedes the formation of the pollen tube, was studied. During this period, pollen grain is being hydrated, abruptly increasing its volume, and it passes from the resting state to active metabolism. Fluorescent methods have made it possible to reveal reactive oxygen species in the cytoplasm and inner layer of the pollen wall, intine. The cytoplasmic reactive oxygen species were mostly found in mitochondria, while extracellular ones were localized in aperture zones of intine, as well as in the solution surrounding pollen grains in vitro. The content of extracellular reactive oxygen species decreased after superoxide dismutase (100 units per ml) and diphenylene iodonium (100 µM), which indicates NADPH oxidase as one of possible producent of them. In conditions of suppression of extracellular reactive oxygen species production (100 µM diphenilene iodonium) or their promoted removal (after addition of 10 to 100 µM ascorbic acid), the number of germinating pollen grains increased. This effect disappeared after further increase in the concentration of the listed reagents. The result is evidence of the significance of processes of generation/removal of extracellular reactive oxygen species for pollen germination.
ISSN:1062-3604
1608-3326
DOI:10.1134/S1062360409060034