Transfer of genetic constructions through the transplacental barrier into mice embryos

Genetic modification of mammalian embryos is an important way to model various changes in human development; also, it is an instrument for studying the functions of certain genes in mammals. Using our own experience in developing modes of delivery of genetic constructions to mammals in a nonviral wa...

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Bibliographic Details
Published in:Russian journal of developmental biology 2010, Vol.41 (2), p.71-76
Main Authors: Efremov, A. M, Buglaeva, A. O, Orlov, S. V, Burov, S. V, Ignatovich, I. A, Dizhe, E. B, Shavva, V. S, Perevozchikov, A. P
Format: Article
Language:English
Subjects:
Animal Anatomy
Biomedical and Life Sciences
Developmental Biology
Experimental Embriology
Histology
hydrodynamic injections
Life Sciences
luliberin derivative
mammalian embryos
Morphology
transfer of genetic constructions
transplacental barrier
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Summary:Genetic modification of mammalian embryos is an important way to model various changes in human development; also, it is an instrument for studying the functions of certain genes in mammals. Using our own experience in developing modes of delivery of genetic constructions to mammals in a nonviral way, we present here data on the delivery of a eukaryotic expression vector to mice embryos through the transpla-cental barrier with the use of hydrodynamic intravenous injections of DNA-hybrid peptide complexes to pregnant females. The peptide has a cationic part for interaction with DNA and includes a ligand structure towards receptors of the releasing factor of luteinizing hormone (RFLH, luliberin). Advantages of the suggested method are simplicity, economy, nonimmunogenicity for females, and the ability to multiply repeat the procedure. On the basis of the method, systemic gene delivery into tissues of mammalian embryos may be developed.
ISSN:1062-3604
1608-3326
DOI:10.1134/S1062360410020025