A second generation universal microarray for subtyping influenza a virus

The microchip for influenza A subtyping working on the “one spot-one subtype” principle was developed. Each spot contains a set of oligonucleotide probes specific for particular subtypes of hemagglutinin, neuraminidase and matrix protein (influenza A marker). Reliability of the proposed chip is the...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2012-11, Vol.38 (6), p.599-604
Main Authors: Kostina, E. V., Ryabinin, V. A., Maksakova, G. A., Sinyakov, A. N.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Life Sciences
Organic Chemistry
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Summary:The microchip for influenza A subtyping working on the “one spot-one subtype” principle was developed. Each spot contains a set of oligonucleotide probes specific for particular subtypes of hemagglutinin, neuraminidase and matrix protein (influenza A marker). Reliability of the proposed chip is the same as for the full-size microchip for separate hemagglutinin and neuraminidase typing which was created in our group earlier. The image was visualized by labeling the analyzed nucleic acid by either fluorescent dye or biotin with the following fixation in streptavidin-gold nanoparticles and development by silver precipitation. In the second case, the image was analyzed using an ordinary scanner that essentially simplifies influenza A subtyping.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162012060052