Affinity hemosorbents based on aromatic peptides for the binding of immunoglobulins G

• Published in: Russian journal of bioorganic chemistry 2015-09, Vol.41 (5), p.494-499
• Main Authors: Levashov, P. A., Ovchinnikova, E. D., Fried, D. A., Azmuko, A. A., Afanasjeva, M. I., Kotkina, T. I., Afanasjeva, O. I., Adamova, I. Yu, Pokrovsky, S. N.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Life Sciences; Organic Chemistry
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162015040081

Hemocompatible affinity sorbents based on the polysaccharide matrix and WY, WTY, and WNY ligands for the binding of human immunoglobulins G (IgG) have been synthesized. The characteristics of the sorbents such as the binding of total IgG and IgG subclasses have been compared. It has been found that the novel sorbents easily extract IgG from the blood plasma, and the WNY-based sorbent is 1.5 times more effective than the others in binding IgG subclass 3 (IgG3). The physicochemical characteristics of the IgG binding have been determined. The desorption constants for IgG are 10 ± 3, 28 ± 4, and 13 ± 3 µM for the sorbents based on WY, WTY, and WNY peptides, respectively. The maximum sorption capacity for IgG is 43 ± 2, 45 ± 3, and 46 ± 3 mg of IgG per 1 mL of sorbent for WY-, WTY-, and WNY-based sorbents, respectively. It has been shown that the sorbents are compatible with blood and are suitable for medicinal applications.