Spectroscopic and anticancer studies on new synthesized copper(II) and manganese(II) complexes with 1,2,4-triazines thiosemicarbazide

• Published in: Russian journal of general chemistry 2015-03, Vol.85 (3), p.692-707
• Main Authors: Refat, Moamen S., El-Deen, I. M., El-Garib, M. S., Abd El-Fattah, W.
• Format: Article
• Language: English
• Subjects: Chemistry; Chemistry and Materials Science; Chemistry/Food Science
• ISSN: 1070-3632; 1608-3350
• DOI: 10.1134/S1070363215030299

Binuclear Cu(II) and Mn(II) newly transition metal complexes of the 5-benzylidene-3-(4-chlorophenyl)-6-oxo-5,6-dihydro-1 H -[1,2,4]triazine-2-carbothioic acid amide (HL 1 ) and 5-(3-bromo-4-methoxybenzylidene)-3-(4-chloro-phenyl)-6-oxo-5,6-dihydro-1 H -[1,2,4]triazine-2-carbothioic acid amide (HL 2 ) derived from the condensation of oxazolinone with thiosemicarbazide have been prepared. The structural elucidation was discussed upon elemental analyses and molar conductivity, as well as from mass, 1 H NMR, IR, UV-Vis spectral studies. From the analytical, spectroscopic and thermal data, the stoichiometry of the mentioned complexes was found to be 1: 2 (metal: ligand). The molar conductance data revealed that all the metal chelates are non-electrolytes and the sulphato ions existing inside of the coordination sphere. The thermal stabilities of these complexes were studied by thermogravimetric (TG-DTG/DTA) and the decomposition steps of these complexes are investigated. The kinetic thermodynamic parameters such as the energy of activation ( E *), pre-exponential factor ( A ), activation entropy (Δ S *), activation enthalpy (Δ H *) and free energy of activation (Δ G *) have been calculated. Inhibitory activity against breast carcinoma cells (MCF-7 cell line), hepatocellular carcinoma cells (HePG-2 cell line) and colon carcinoma cells (HCT cell line) were tested by using different concentrations of the samples (50, 25, 12.5, 6.25, 3.125, and 1.56 μg) and cell viability (%) was determined by colorimetric method. The drug doxrubcin was used as standard.