Spontaneous aneuploidy level in blood cells of fertile females

In this study, we presented results of molecular cytogenetic assay of blood cells of fertile women of reproductive age. Cultivated and uncultivated cells and two sets of FISH probes with direct and indirect labeling were used. The middle level of aneuploidy for four chromosomes and statistical limit...

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Bibliographic Details
Published in:Cell and tissue biology 2009, Vol.3 (4), p.397-402
Main Authors: Zotova, N. V., Markova, E. V., Lebedev, I. N., Svetlakov, A. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Life Sciences
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Summary:In this study, we presented results of molecular cytogenetic assay of blood cells of fertile women of reproductive age. Cultivated and uncultivated cells and two sets of FISH probes with direct and indirect labeling were used. The middle level of aneuploidy for four chromosomes and statistical limits for aneuploidy detection were estimated. Aneuploidy determined with direct multicolor FISH in cultivated lymphocytes varied from 0.1 to 1.3%. The middle level of aneuploidy for all four chromosomes (13, 18, 21, and X) was 1.39%. The limit of mutation detection was 3.4%. We found differences in results with direct and indirect labeling. It was shown that the cultivation process influenced the level of aneuploidy. The absolute error in FISH technique was 0.13% and the relative error was 4.08%.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X09040129