The effect of glutoxim on Na+ transport in frog skin: The role of cytoskeleton

Using the voltage-clamp technique, the possible implication of cytoskeleton in the effect of glutoxim, a pharmacological analog of oxidized glutathione (GSSG), on Na + transport in the skin of frog Rana temporaria was investigated. It was shown for the first time that skin preincubation with nocodaz...

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Bibliographic Details
Published in:Cell and tissue biology 2012-05, Vol.6 (3), p.248-253
Main Authors: Melnitskaya, A. V., Krutetskaya, Z. I., Lebedev, O. E., Butov, S. N., Krutetskaya, N. I., Antonov, V. G.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Life Sciences
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Summary:Using the voltage-clamp technique, the possible implication of cytoskeleton in the effect of glutoxim, a pharmacological analog of oxidized glutathione (GSSG), on Na + transport in the skin of frog Rana temporaria was investigated. It was shown for the first time that skin preincubation with nocodazole, a microtubular disrupter; cytochalasin D, actin filament disrupter; or protein phosphatase PP1/PP2A inhibitor calyculin A significantly decreased the stimulatory effect of glutoxim on Na + transport. The results suggest the involvement of microtubules and microfilaments in the regulatory effect of glutoxim on Na + transport in frog skin and that reorganization of actin filaments or microtubules leads to inhibition of the stimulatory effect of glutoxim on Na + transport in frog skin epithelia.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X1203008X