Antioxidant action on the level of reactive oxygen species in normal and transformed fibroblasts

The level of reactive oxygen species (ROS) in normal (3T3) and transformed (3T3-SV40) murine fibroblasts treated with antioxidants for 15 min was studied using a carboxy-H 2 DCFDA fluorescent probe. It was shown that N-acetylcysteine (NAC) decreased the ROS level in both cell types. Antioxidant alph...

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Bibliographic Details
Published in:Cell and tissue biology 2014, Vol.8 (1), p.33-37
Main Authors: Lyublinskaya, O. G., Kirpichnikova, K. M., Gamaley, I. A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Life Sciences
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Summary:The level of reactive oxygen species (ROS) in normal (3T3) and transformed (3T3-SV40) murine fibroblasts treated with antioxidants for 15 min was studied using a carboxy-H 2 DCFDA fluorescent probe. It was shown that N-acetylcysteine (NAC) decreased the ROS level in both cell types. Antioxidant alpha-lipoic acid (ALA) and its reduced form dihydrolipoic acid (DHLA) caused a pro-oxidant effect. ALA and DHLA in the concentration range from 0.1–1.25 mM increased the ROS level in a dose-dependent manner in both cell types. The ability of ALA and DHLA to activate hydrogen peroxide production is discussed.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X14010088