Peculiar properties of some components in a plant cell vacuole morphological structure revealed by confocal microscopy

• Published in: Cell and tissue biology 2015-09, Vol.9 (5), p.406-414
• Main Authors: Nurminsky, V. N., Rakevich, A. L., Martynovich, E. F., Ozolina, N. V., Nesterkina, I. S., Kolesnikova, E. V., Pilipchenko, A. A., Salyaev, R. K., Chernyshov, M. Yu
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cell Biology; Life Sciences
• ISSN: 1990-519X; 1990-5203
• DOI: 10.1134/S1990519X15050090

Results of investigation of peculiar properties related to the structure of plant cell vacuolar membranes are discussed. This investigation was conducted with the aid of confocal microscopy, which allowed us, in the process of scanning, to reveal nanotubes and vesicules in the preparations of isolated vacuoles. Such membrane nanotubes were observed both inside and outside the vacuoles, and, in the case of scanning intermittently at equal time intervals, transition of vesicules with a membrane nanotube. Furthermore, scanning of isolated vacuoles was conducted at various distances from the glass basement. Each time, in the upper part of an isolated vacuole lying on the basement, we observed a large segment of vacuolar membrane and registered the effect of highly intense fluorescing of some of the membrane segments. Distributions of laurdan fluorescence generalized polarization (GP) values for the vacuolar membrane on the whole and for the intensely fluorescing membrane segments have been obtained. It was discovered that the microviscosity of intensely fluorescing membrane segments essentially differs from the microviscosity of the rest part of the membrane.