Gastroprotective effects of montelukast and Nigella sativa oil against corticosteroid-induced gastric damage: they are much more than antiasthmatic drugs

• Published in: Canadian journal of physiology and pharmacology 2017-06, Vol.95 (6), p.714-720
• Main Authors: Rizk, Fatma H, Ibrahim, Marwa A.A, Abd-Elsalam, Marwa M, Soliman, Nema A, Abd-Elsalam, Sherief M
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; Adrenal Cortex Hormones - adverse effects; Animals; Anti-Asthmatic Agents - pharmacology; Antigens; Asthma; Care and treatment; Cells; Complications and side effects; Corticosteroid drugs; Corticosteroids; Cytoprotection - drug effects; dexamethasone; Dexamethasone - adverse effects; dexaméthasone; dommages gastriques; Drug Interactions; gastric damage; Health aspects; Male; Medical treatment; Montelukast; montélukast; Nigella sativa; Nigella sativa - chemistry; Oxidative Stress - drug effects; Peroxidase - metabolism; Pharmacology; Physiology; Plant Oils - pharmacology; Quinolines - pharmacology; Rats; Rats, Wistar; Stomach - cytology; Stomach - drug effects; Stomach - metabolism; Stomach diseases
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2016-0374

Corticosteroids are used to treat a variety of diseases like bronchial asthma. However, long-term corticosteroids have a gastric ulcerogenic potential. Montelukast (MTK) and Nigella sativa oil (NSO) are used in treatment of bronchial asthma. Previous studies showed that MTK and NSO had gastroprotective effects in other models of gastric ulcer. The present study assesses synergistic gastroprotective effects of both drugs in dexamethasone (DXM)-induced gastric damage. Fifty male rats were divided into 5 groups: normal control (I), DXM group (II), MTK + DXM group (III), NSO + DXM group (IV), MTK + NSO + DXM group (V). After 7 days, stomachs were removed for biochemical analysis and histological examinations. Significant increases in malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, myeloperoxidase (MPO) activity, and proliferating cell nuclear antigen (PCNA) positive cells, with significant decreases in mucus secretion were detected in DXM-treated group compared with group I. Meanwhile, significant decreases of MDA level, MPO activity, and PCNA positive cells and significant increases in mucus secretion were detected in treated groups compared with group II. SOD activity significantly decreased in group V compared with group II. We could conclude that administration of either MTK or NSO or both with DXM counteracts DXM-induced gastric lesions.