Observations on cation-binding characteristics and nuclear magnetic resonance spectra of the N-carboxymethyl and N-acetyl analogs of heparin

Based on the observed paramagnetic relaxation effects of Cu 2+ and Fe 3+ ions and the chemical shift displacements induced by Ca 2+ ions, nuclear resonance spectroscopy has been used to compare cation-binding characteristics of heparin with those of its N-carboxymethyl and N-acetyl analogs and relat...

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Bibliographic Details
Published in:Canadian journal of chemistry 1990-10, Vol.68 (10), p.1740-1745
Main Authors: Rej, Rabindra N, Holme, Kevin R, Perlin, Arthurs. S
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates
Fundamental and applied biological sciences. Psychology
Holosides
Other biological molecules
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Summary:Based on the observed paramagnetic relaxation effects of Cu 2+ and Fe 3+ ions and the chemical shift displacements induced by Ca 2+ ions, nuclear resonance spectroscopy has been used to compare cation-binding characteristics of heparin with those of its N-carboxymethyl and N-acetyl analogs and related polyanions. Heparin is unique in exhibiting a high affinity for all three cations. Among the other polymers, binding selectivities differ notably. Such factors as the incidence and distribution of anionic charges are discussed. The preparation of the N-carboxymethyl analog of heparin is described, and 1 H and 13 C nuclear magnetic resonance spectroscopic data are reported for it, as well as for the N-acetyl analog. Keywords: heparin, N-carboxymethyl analog, N-acetyl analog, cation binding, ion-selectivity, anionic charge distribution.
ISSN:0008-4042
1480-3291
DOI:10.1139/v90-270