Home-built automated system for microorganisms identification based on laser scattering: A low-cost design and construction using a CNC milling and pattern recognition

• Published in: The European physical journal. ST, Special topics Special topics, 2020-11, Vol.229 (17-18), p.2833-2841
• Main Authors: Kitaoka, Natália A. A., Benevides, Clayton A., Tenório, Rômulo P.
• Format: Article
• Language: English
• Subjects: Atomic; Classical and Continuum Physics; Condensed Matter Physics; Dynamics and Functionality; Evolving Soft Matter: Shape; Materials Science; Measurement Science and Instrumentation; Molecular; Optical and Plasma Physics; Physics; Physics and Astronomy; Regular Article
• ISSN: 1951-6355; 1951-6401
• DOI: 10.1140/epjst/e2020-900212-x

The identification of microorganisms between different types and species has great importance in hospitals, food industry and pharmaceutical companies. In recent works, it was found that different microorganisms colony species, which show similar visual morphology, provide a distinctive forward-scattering pattern when struck by a laser beam. The produced pattern was used as a microorganism signature and identification. Comparing with the current methods of microorganism identification, which are laborious, time-consuming, and costly to implement, the identification based on the image scattering pattern recognition sounds very appealing. In this work, we constructed a non-expensive portable laser scattering image device, based on the acquisition of colony laser scattering images and pattern recognition, to classify, distinguish and identify among microorganisms. We tested our system with three model microorganisms, Bacillus subtilis , Staphylococcus aureus and Saccharomyces cerevisiae . Our results showed a recovery of 92% on the accuracy in the identification of the microorganisms.