Molecularly flat films of linear polyions and proteins obtained by the alternate adsorption method

Alternately assembled films of poly(ethyleneimine) (PEI) and poly(styrenesulfonate) (PSS), as examined by non-contact atomic force microscopy (AFM), provided molecularly flat surfaces with height difference of several Å. Similar observations on alternate protein films of PEI-glucose oxidase, PEI-fer...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 1997-12, Vol.36 (12A), p.L1608-L1611
Main Authors: ONDA, M, LVOV, Y, ARIGA, K, KUNITAKE, T
Format: Article
Language:English
Subjects:
Applied sciences
Exact sciences and technology
Organic polymers
Physicochemistry of polymers
Properties and characterization
Structure, morphology and analysis
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Summary:Alternately assembled films of poly(ethyleneimine) (PEI) and poly(styrenesulfonate) (PSS), as examined by non-contact atomic force microscopy (AFM), provided molecularly flat surfaces with height difference of several Å. Similar observations on alternate protein films of PEI-glucose oxidase, PEI-ferritin and PSS-peroxidase showed that films with outermost protein layers gave well-packed island structures and that the size of the island and the film roughness were related to the size of proteins used. Subsequent adsorption of a polyion layer decreased the surface roughness of protein layers significantly probably due to bridging of protein molecules by polyion chains.
ISSN:0021-4922
1347-4065
DOI:10.1143/jjap.36.L1608