Simple Method of Confirming Wet Environment for Soft X-ray Microscopy to Observe Hydrated Biological Specimens

Observations of hydrated specimens were carried out by soft X-ray microscopy using a newly developed wet-environment sample holder. The sample holder consisted of two silicon nitride membranes, one of which was printed with a carbon-based spacer. A reverse-contrast image of the spacer was obtained a...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2012-09, Vol.51 (9), p.092401-092401-5
Main Authors: Takaba, Kenichiro, Hoshino, Masato, Aoki, Sadao
Format: Article
Language:English
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Summary:Observations of hydrated specimens were carried out by soft X-ray microscopy using a newly developed wet-environment sample holder. The sample holder consisted of two silicon nitride membranes, one of which was printed with a carbon-based spacer. A reverse-contrast image of the spacer was obtained at two different wavelengths on opposite sides of the carbon K absorption edge. By obtaining a reverse-contrast image of the spacer used in the sample holder, one can confirm the wet environment of the specimen. The general principles and efficiency of this novel method are discussed.
ISSN:0021-4922
1347-4065
DOI:10.1143/JJAP.51.092401