Nutrient regulation of PKCε is mediated by leucine, not insulin, in skeletal muscle

• Published in: American journal of physiology: endocrinology and metabolism 2005-10, Vol.289 (4), p.E684-E694
• Main Authors: Vary, Thomas C., Goodman, Stacy, Kilpatrick, Laurie E., Lynch, Christopher J.
• Format: Article
• Language: English
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.00613.2004

Nutrients enhance signaling pathways involved in skeletal muscle growth through an increased rate of protein synthesis. These studies have led to an understanding of the potential role of the mammalian target of rapamycin (mTOR) in this process. However, activation of mTOR cannot account for all the stimulatory effects of nutrients. The purpose of these experiments was to examine the effect of nutrients on the cellular distribution and activation state of novel PKC isoforms (PKCε and PKCδ) in the gastrocnemius of rats by use of modification state-dependent phosphopeptide-specific antibodies. The phosphorylation of PKCε on the catalytic domain autophosphorylation site (Ser 729 ) was elevated during feeding and then returned to basal levels when the feeding period ended. Meal feeding augmented the phosphorylation of the downstream effectors of mTOR, namely S6K1 and 4E-BP1. In contrast, the phosphorylation of PKCδ on either the catalytic domain autophosphorylation site (Ser 643 ) or activation loop site (Thr 505 ) was unaffected. Similar results were obtained when animals were given leucine either acutely via gavage or chronically by dietary supplementations. The effect of leucine was not mimicked by injecting animals with insulin but could be induced by gavage with norleucine, a structural analog of leucine that does not increase plasma insulin concentration. Thus rises in insulin secondary to meal intake or leucine gavage are probably not responsible for increased phosphorylation of PKCε in response to meal feeding. Elevating the leucine concentration stimulated the phosphorylation of PKCε in gastrocnemius from perfused hindlimb and caused a shift in the distribution of PKCε from the membrane fraction to the cytosolic fraction. The results indicate that leucine leads to an activation (autophosphorylation) and subcellular redistribution of PKCε, but not PKCδ, in gastrocnemius both in vivo and in vitro. Furthermore, activation of the mTOR signaling pathway above basal conditions does not appear to be necessary to induce phosphorylation or translocation of PKCε, suggesting that multiple signaling pathways become activated with leucine.