Lactate clamp: a method to measure lactate utilization in vivo

Diabetes Pharmacology Unit, Novartis Pharmaceuticals Corporation, Summit, New Jersey 07901 A lactate clamp method has been developed to quantify the whole body lactate utilization in conscious, unstressed rats. Dichloroacetate (DCA), a known lactate utilization enhancer, was used to validate the met...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology: endocrinology and metabolism 1998-10, Vol.275 (4), p.E729-E733
Main Authors: Gao, Jiaping, Islam, Mohammad A, Brennan, Christine M, Dunning, Beth E, Foley, James E
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Diabetes Pharmacology Unit, Novartis Pharmaceuticals Corporation, Summit, New Jersey 07901 A lactate clamp method has been developed to quantify the whole body lactate utilization in conscious, unstressed rats. Dichloroacetate (DCA), a known lactate utilization enhancer, was used to validate the method. Fasting blood lactate concentrations before the clamps were identical for DCA-treated (1 mmol/kg) and control groups (1.65 ± 0.37 vs. 1.65 ± 0.19 mM). The animals received a primed continuous lactate infusion for 90 min at variable rates to clamp the blood lactate concentration at 2 mM. The steady-state (60-90 min) lactate infusion rate, which represents the whole body lactate utilization in DCA-treated animals, was 144% higher than that in the control animals (13.2 ± 1.0 vs. 5.4 ± 1.1 mg · kg 1 · min 1 ; P  
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.1998.275.4.e729