Stroking human jejunal mucosa induces 5-HT release and Cl - secretion via afferent neurons and 5-HT 4 receptors

5-Hydroxytryptamine (5-HT) release and neural reflex pathways activated in response to mucosal stroking were investigated in muscle-stripped human jejunum mounted in modified Ussing chambers. The mucosa was stroked with a brush at 1/s for 1-10 s. Mucosal stroking resulted in a significant increase i...

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Published in:American journal of physiology: Gastrointestinal and liver physiology 1999-09, Vol.277 (3), p.G515-G520
Main Authors: Kellum, John M, Albuquerque, Francisco C, Stoner, Michael C, Harris, R Paul
Format: Article
Language:English
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Summary:5-Hydroxytryptamine (5-HT) release and neural reflex pathways activated in response to mucosal stroking were investigated in muscle-stripped human jejunum mounted in modified Ussing chambers. The mucosa was stroked with a brush at 1/s for 1-10 s. Mucosal stroking resulted in a significant increase in the concentration of 5-HT in the mucosal bath within 5 min. It also was associated with a reproducible positive change (Δ) in short-circuit current ( I ), which was abolished by inhibitors of chloride secretion. Capsaicin and hexamethonium significantly inhibited the Δ I but not the release of 5-HT. The Δ I was inhibited by TTX but not by atropine. It was also inhibited by the 5-HT receptor antagonist tropisetron (10 μM) and the 5-HT receptor antagonist SDZ-205-557 (10 μM) but not by preferential antagonists of 5-HT , 5-HT , or 5-HT receptors. These results suggest that mucosal stroking induces release of mucosal 5-HT, which activates a 5-HT receptor on enteric sensory neurons, evoking a neuronal reflex that stimulates chloride secretion.
ISSN:0193-1857
1522-1547
DOI:10.1152/ajpgi.1999.277.3.G515