NO metabolites accumulate in erythrocytes in proportion to carbon dioxide and bicarbonate concentration

Department of Physiology, New York Medical College, Valhalla, New York 10595 It is not known whether the ratio between the concentrations of NO metabolites (NOx) in plasma (pNOx) and in erythrocytes (eNOx) is constant or correlates with chemical parameters of the blood. We measured pH, P O 2 , and P...

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Published in:American journal of physiology. Heart and circulatory physiology 2000-08, Vol.279 (2), p.H852-H856
Main Authors: Recchia, Fabio A, Vogel, Traci R, Hintze, Thomas H
Format: Article
Language:English
Subjects:
Animals
Aorta
Bicarbonates - blood
Carbon Dioxide - blood
Dogs
Endothelium, Vascular - physiology
Erythrocytes - metabolism
Models, Cardiovascular
Nitric Oxide - physiology
Nitrogen Oxides - blood
Veins
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Summary:Department of Physiology, New York Medical College, Valhalla, New York 10595 It is not known whether the ratio between the concentrations of NO metabolites (NOx) in plasma (pNOx) and in erythrocytes (eNOx) is constant or correlates with chemical parameters of the blood. We measured pH, P O 2 , and P CO 2 and calculated bicarbonate concentration in 19 blood samples from the aorta, coronary sinus, and leg veins of 7 dogs. Erythrocytes were then separated by centrifugation and lysed with distilled water, and the lysate was ultrafiltered with a molecular cutoff of 50 kDa to remove the hemoglobin. NOx were measured in plasma and in the ultrafiltrate. NOx concentration was higher in erythrocytes, with eNOx/pNOx ranging from 4.38 to 14.60. Linear and significant correlations were found between the natural logarithm of eNOx/pNOx and P CO 2 ( r  = 0.70,  P
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.2000.279.2.h852