Anti-neutrophil chemokine preserves alveolar development in hyperoxia-exposed newborn rats

• Published in: American journal of physiology. Lung cellular and molecular physiology 2001-08, Vol.281 (2), p.336-L344
• Main Authors: Auten, Richard L., Jr, Mason, S. Nicholas, Tanaka, David T, Welty-Wolf, Karen, Whorton, Mary H
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - growth & development; Antibodies - pharmacology; Bromodeoxyuridine - metabolism; Bronchoalveolar Lavage Fluid - cytology; Cell Division; Chemokine CXCL1; Chemokines - analysis; Chemokines, CXC; Chemotactic Factors - immunology; DNA - metabolism; Growth Substances - immunology; Hyperoxia - pathology; Hyperoxia - physiopathology; Intercellular Signaling Peptides and Proteins; Leukocyte Count; Lung - metabolism; Lung - pathology; Neutrophils - drug effects; Neutrophils - pathology; Proliferating Cell Nuclear Antigen - metabolism; Pulmonary Alveoli - drug effects; Pulmonary Alveoli - growth & development; Pulmonary Alveoli - pathology; Rats; Survival Analysis; Weight Gain
• ISSN: 1040-0605; 1522-1504
• DOI: 10.1152/ajplung.2001.281.2.l336

1  Division of Neonatal Medicine, Department of Pediatrics, Neonatal-Perinatal Research Institute, and 3  Division of Pulmonary and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham 27710; and 2  Comprehensive Center for Inflammatory Disorders, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 Inflammation may contribute to lung injury and impaired alveolar development in bronchopulmonary dysplasia. We treated hyperoxia-exposed newborn rats with antibodies to the neutrophil chemokine cytokine-induced neutrophil chemoattractant-1 (CINC-1) during 95% O 2 exposure to reduce adverse effects of hyperoxia-induced inflammation on lung development. Rats were exposed at birth to air, 95% O 2 , or 95% O 2  + anti-CINC-1 (injected on days 3  and 4 ). Bromodeoxyuridine (BrdU) was injected 6 h before death. Anti-CINC-1 treatment improved weight gain but not survival at day 8 . Anti-CINC-1 reduced bronchoalveolar lavage neutrophils at day 8  to levels equal to air controls. Total detectable lung CINC-1 was reduced to air control levels. Lung compliance was improved by anti-CINC-1, achieving air control levels in the 10-µg anti-CINC-1 group. Anti-CINC-1 preserved proliferating cell nuclear antigen expression in airway epithelium despite 95% O 2 exposure. BrdU incorporation was depressed by hyperoxia but preserved by anti-CINC-1 to levels similar to air control. Alveolar volume and surface density were decreased by hyperoxia but preserved by anti-CINC-1 to levels equal to air control. Blockade of neutrophil influx in newborns may avert early lung injury and avoid alveolar developmental arrest that contributes to bronchopulmonary dysplasia. chemotactic factors; proliferation; bromodeoxyuridine; proliferating cell nuclear antigen