Expression of CD44 in kidney after acute ischemic injury in rats

George M. O'Brien Kidney and Urological Diseases Center, Renal Division, Departments of Medicine and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110 De novo CD44 and ligand expression at wound margins accompanies cellular proliferation and migrat...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2000-01, Vol.278 (1), p.247-R254
Main Authors: Lewington, Andrew J. P, Padanilam, Babu J, Martin, Daniel R, Hammerman, Marc R
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Hyaluronan Receptors - genetics
Hyaluronan Receptors - metabolism
Hyaluronic Acid - metabolism
Ischemia - metabolism
Kidney - metabolism
Kidney Tubules, Proximal - metabolism
Kidney Tubules, Proximal - physiopathology
Male
Osteopontin
Rats
Rats, Sprague-Dawley
Regeneration
Renal Circulation
RNA, Messenger - metabolism
Sialoglycoproteins - metabolism
Time Factors
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:George M. O'Brien Kidney and Urological Diseases Center, Renal Division, Departments of Medicine and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110 De novo CD44 and ligand expression at wound margins accompanies cellular proliferation and migration that effect repair of injured mucosal and vascular endothelial tissues. To determine whether CD44 could play a role in recovery from acute ischemic renal injury, we characterized its renal expression and those of two of its ligands, hyaluronic acid and osteopontin. Although no expression is detectable in nonischemic kidneys, several mRNAs for CD44 are present within 1 day after injury. CD44 mRNA is expressed in proximal tubules undergoing repair. CD44 peptide is present in basal and lateral cell membranes. Hyaluronic acid is normally expressed in the interstitium of the renal papilla only. By 1   day postischemia, hyaluronic acid can be detected, in addition, in the interstitium surrounding regenerating tubules. Osteopontin, not normally expressed in the renal proximal tubule, is expressed in regenerating tubules by 3 days after induction of acute ischemic injury. Immunoreactive osteopontin peptide continues to be localized in those tubules still undergoing repair for as long as 7 days after the injury. Our data are consistent with a role for CD44-ligand interactions in the regenerating proximal tubule participating in the process of recovery after ischemic injury. acute renal failure; hyaluronic acid; matrix; osteopontin; arginine-glycine-aspartate peptides
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.2000.278.1.R247