Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

1  Laboratory, 5  Medicine and 8  Surgery Services, James A. Haley Veterans Hospital, and Departments of 2  Biochemistry and Molecular Biology, 6  Internal Medicine, 7  Physiology and Biophysics, and 3  Surgery, University of South Florida, and 4  University of South Florida Cardiac Hormone Center,...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2000-03, Vol.278 (3), p.770-R780
Main Authors: Gower, William R., Jr, Salhab, Khaled F, Foulis, Wendy L, Pillai, Nirmala, Bundy, Jason R, Vesely, David L, Fabri, Peter J, Dietz, John R
Format: Article
Language:English
Subjects:
Animals
Atrial Natriuretic Factor - genetics
Fasting - physiology
Gene Expression Regulation - physiology
Immunohistochemistry
Male
Polymerase Chain Reaction
Rats
Rats, Sprague-Dawley
RNA, Messenger - analysis
Stomach - physiology
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Summary:1  Laboratory, 5  Medicine and 8  Surgery Services, James A. Haley Veterans Hospital, and Departments of 2  Biochemistry and Molecular Biology, 6  Internal Medicine, 7  Physiology and Biophysics, and 3  Surgery, University of South Florida, and 4  University of South Florida Cardiac Hormone Center, Tampa, Florida 33612 Atrial natriuretic peptide (ANP) gene expression was localized in the rat gastric antrum using immunohistochemistry and in situ hybridization to mucosal cells in the lower portion of the antropyloric glands. Colocalization of immunoreactive ANP, long-acting natriuretic peptide, i.e., proANP-(1 30), and serotonin in these cells identified them to be enterochromaffin cells. Fasting for 72 h in 8-mo-old (adult) rats produced a significant ( P   BNP >> CNP, suggesting that these receptors were functional. We conclude that fasting decreases ANP prohormone mRNA and its gene products, long-acting natriuretic peptide, and ANP in the antrum of adult rats. natriuretic peptide receptors; stomach; enterochromaffin cells; reverse transcription-polymerase chain reaction
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.2000.278.3.r770