Loading…

Leukotriene B 4 promotes reactive oxidant generation and leukocyte adherence during acute hypoxia

Acute systemic hypoxia produces rapid leukocyte adherence in the rat mesenteric microcirculation, although the underlying mechanisms are not fully known. Hypoxia is known to increase reactive oxygen species (ROS) generation, which could result in formation of the lipid inflammatory mediator leukotri...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied physiology (1985) 2001-09, Vol.91 (3), p.1160-1167
Main Authors: Steiner, Dawn R. S., Gonzalez, Norberto C., Wood, John G.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute systemic hypoxia produces rapid leukocyte adherence in the rat mesenteric microcirculation, although the underlying mechanisms are not fully known. Hypoxia is known to increase reactive oxygen species (ROS) generation, which could result in formation of the lipid inflammatory mediator leukotriene B 4 (LTB 4 ). The goal of this study was to examine the role of LTB 4 in hypoxia-induced microvascular alterations. Using intravital microscopy, we determined the effect of the LTB 4 antagonist, LTB 4 -dimethyl amide (LTB 4 -DMA), on ROS generation and leukocyte adherence in mesenteric venules during hypoxia. Exogenous LTB 4 increased ROS generation to 144 ± 8% compared with control values and also promoted leukocyte adherence. These responses to LTB 4 were blocked by pretreating the mesentery with LTB 4 -DMA. Leukopenia did not significantly attenuate the LTB 4 -induced increase in ROS generation (142 ± 12.1%). LTB 4 -DMA substantially, though not completely, reduced hypoxia-induced ROS generation from 66 ± 18% to 11 ± 4% above control values. Hypoxia-induced leukocyte adherence was significantly attenuated by LTB 4 -DMA. Our results support a role for LTB 4 in the mechanism of hypoxia-induced ROS generation and leukocyte adherence in the rat mesenteric microcirculation.
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.2001.91.3.1160