Phospholipids modulate the biophysical properties and vasoactivity of PACAP-(138)

• Published in: Journal of applied physiology (1985) 2002-10, Vol.93 (4), p.1377-1383
• Main Authors: Tsueshita, Takaya, Gandhi, Salil, Onyuksel, Hayat, Rubinstein, Israel
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Arterioles - drug effects; Biological and medical sciences; Biophysical Phenomena; Biophysics; Circulatory system; Cricetinae; Fundamental and applied biological sciences. Psychology; Glycerolipids, phospholipids; Lipids; Mesocricetus; Micelles; Molecular Conformation; Neuropeptides - chemistry; Neuropeptides - metabolism; Neuropeptides - pharmacology; Osmolar Concentration; Other biological molecules; Peptide Fragments - chemistry; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; Peptides; Phospholipids - physiology; Pituitary Adenylate Cyclase-Activating Polypeptide; Pressure; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; Receptors, Pituitary Hormone - antagonists & inhibitors; Receptors, Vasoactive Intestinal Peptide - antagonists & inhibitors; Surface Tension; Vasoactive Intestinal Peptide - pharmacology; Vasodilator Agents - chemistry; Vasodilator Agents - metabolism; Vasodilator Agents - pharmacology; Vasomotor System - drug effects
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00277.2002

Departments of 1  Biopharmaceutical Sciences, 2  Bioengineering, and 3  Medicine, University of Illinois at Chicago, and 4  Chicago Veterans Afffairs Health Care System, West Side Division, Chicago, Illinois 60612 The purpose of this study was to elucidate the interactions between pituitary adenylate cyclase-activating peptide (PACAP)-(1 38) and phospholipids in vitro and to determine whether these phenomena modulate, in part, the vasorelaxant effects of the peptide in the intact peripheral microcirculation. We found that the critical micellar concentration of PACAP-(1 38) was 0.4-0.9 µM. PACAP-(1 38) significantly increased the surface tension of a dipalmitoylphosphatidylcholine monolayer and underwent conformational transition from predominantly random coil in saline to -helix in the presence of distearoyl-phosphatidylethanolamine-polyethylene glycol (molecular mass of 2,000 Da) sterically stabilized phospholipid micelles (SSM) ( P