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Feedback Effects of Horizontal Cell Membrane Potential on Cone Calcium Currents Studied With Simultaneous Recordings
1 Departments of Ophthalmology and Visual Science and 2 Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska Submitted 3 October 2005; accepted in final form 15 December 2005 Horizontal cell (HC) to cone feedback helps establish the center-surround arran...
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Published in: | Journal of neurophysiology 2006-03, Vol.95 (3), p.1992-1995 |
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cites | cdi_FETCH-LOGICAL-c492t-fd291117ccc00271c39711acf602d5a8d7a6ae2b5dfa5e98efeb691c99bcaa2e3 |
container_end_page | 1995 |
container_issue | 3 |
container_start_page | 1992 |
container_title | Journal of neurophysiology |
container_volume | 95 |
creator | Cadetti, Lucia Thoreson, Wallace B |
description | 1 Departments of Ophthalmology and Visual Science and 2 Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska
Submitted 3 October 2005;
accepted in final form 15 December 2005
Horizontal cell (HC) to cone feedback helps establish the center-surround arrangement of visual receptive fields. It has been shown that HC activity influences cone synaptic output by altering the amplitude and voltage dependence of the calcium current ( I Ca ) in cones. In this study, we obtained voltage-clamp recordings simultaneously from cones and HCs to directly control the membrane potential of HCs and thereby measure the influence of HC membrane potential changes on I Ca in adjacent cones. Directly hyperpolarizing voltage clamped HCs produced a negative activation shift and increased the amplitude of I Ca in cones. Both of these effects were abolished by enhancing extracellular pH buffering capacity with HEPES. In contrast, addition of the gap junction blocker, carbenoxolone, did not significantly alter the shifts or amplitude changes in cone I Ca produced by changes in HC membrane potential. These results support the hypothesis that changes in the HC membrane potential alter the voltage dependence and amplitude of cone I Ca by altering extracellular pH levels at the synapse.
Address for reprint requests and other correspondence: W. B. Thoreson, Dept. of Ophthalmology, Univ. of Nebraska Medical Center, Durham Research Center, Omaha, NE 68198-5840 (E-mail: wbthores{at}unmc.edu ) |
doi_str_mv | 10.1152/jn.01042.2005 |
format | article |
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Submitted 3 October 2005;
accepted in final form 15 December 2005
Horizontal cell (HC) to cone feedback helps establish the center-surround arrangement of visual receptive fields. It has been shown that HC activity influences cone synaptic output by altering the amplitude and voltage dependence of the calcium current ( I Ca ) in cones. In this study, we obtained voltage-clamp recordings simultaneously from cones and HCs to directly control the membrane potential of HCs and thereby measure the influence of HC membrane potential changes on I Ca in adjacent cones. Directly hyperpolarizing voltage clamped HCs produced a negative activation shift and increased the amplitude of I Ca in cones. Both of these effects were abolished by enhancing extracellular pH buffering capacity with HEPES. In contrast, addition of the gap junction blocker, carbenoxolone, did not significantly alter the shifts or amplitude changes in cone I Ca produced by changes in HC membrane potential. These results support the hypothesis that changes in the HC membrane potential alter the voltage dependence and amplitude of cone I Ca by altering extracellular pH levels at the synapse.
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Submitted 3 October 2005;
accepted in final form 15 December 2005
Horizontal cell (HC) to cone feedback helps establish the center-surround arrangement of visual receptive fields. It has been shown that HC activity influences cone synaptic output by altering the amplitude and voltage dependence of the calcium current ( I Ca ) in cones. In this study, we obtained voltage-clamp recordings simultaneously from cones and HCs to directly control the membrane potential of HCs and thereby measure the influence of HC membrane potential changes on I Ca in adjacent cones. Directly hyperpolarizing voltage clamped HCs produced a negative activation shift and increased the amplitude of I Ca in cones. Both of these effects were abolished by enhancing extracellular pH buffering capacity with HEPES. In contrast, addition of the gap junction blocker, carbenoxolone, did not significantly alter the shifts or amplitude changes in cone I Ca produced by changes in HC membrane potential. These results support the hypothesis that changes in the HC membrane potential alter the voltage dependence and amplitude of cone I Ca by altering extracellular pH levels at the synapse.
Address for reprint requests and other correspondence: W. B. Thoreson, Dept. of Ophthalmology, Univ. of Nebraska Medical Center, Durham Research Center, Omaha, NE 68198-5840 (E-mail: wbthores{at}unmc.edu )</description><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling - physiology</subject><subject>Cells, Cultured</subject><subject>Feedback - physiology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Membrane Potentials - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Retinal Cone Photoreceptor Cells - physiology</subject><subject>Retinal Horizontal Cells - physiology</subject><subject>Synaptic Transmission - physiology</subject><subject>Urodela</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc2P0zAQxS0EYsvCkSvyCU4pthPH9QUJRVsWaRGIXcTRcvzRuDhx13aA8tfj0mqBA-Jka-Y3T_PmAfAUoyXGlLzcTkuEUUOWBCF6DyxKjVSY8tV9sECo_GvE2Bl4lNIWIcQoIg_BGW5rhhvKFiCvjdG9VF_ghbVG5QSDhZchuh9hytLDzngP35mxj3Iy8EPIZsqu1MMEu1AqnfTKzSPs5hhLK8HrPGtnNPzs8gCv3Tj7XCbDnOBHo0LUbtqkx-CBlT6ZJ6f3HHxaX9x0l9XV-zdvu9dXlWo4yZXVhGOMmVKqOGFY1ZxhLJVtEdFUrjSTrTSkp9pKavjKWNO3HCvOeyUlMfU5eHXU3c39aLQqC0bpxS66Uca9CNKJvzuTG8QmfBWkYU3TsiLw_CQQw-1sUhajS6qc5GhJtKxd0Qa1_wUxQ6vihRewOoIqhpSisXfbYCQOgYrtJH4FKg6BFv7ZnxZ-06cEC_DiCAxuM3xz0YjdsE8u-LDZH7Q4FbXAnJNC1v8m17P3N-Z7LiN3E2Knbf0Tmce_hA</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Cadetti, Lucia</creator><creator>Thoreson, Wallace B</creator><general>Am Phys Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060301</creationdate><title>Feedback Effects of Horizontal Cell Membrane Potential on Cone Calcium Currents Studied With Simultaneous Recordings</title><author>Cadetti, Lucia ; Thoreson, Wallace B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-fd291117ccc00271c39711acf602d5a8d7a6ae2b5dfa5e98efeb691c99bcaa2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling - physiology</topic><topic>Cells, Cultured</topic><topic>Feedback - physiology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Membrane Potentials - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Retinal Cone Photoreceptor Cells - physiology</topic><topic>Retinal Horizontal Cells - physiology</topic><topic>Synaptic Transmission - physiology</topic><topic>Urodela</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cadetti, Lucia</creatorcontrib><creatorcontrib>Thoreson, Wallace B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cadetti, Lucia</au><au>Thoreson, Wallace B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Feedback Effects of Horizontal Cell Membrane Potential on Cone Calcium Currents Studied With Simultaneous Recordings</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>95</volume><issue>3</issue><spage>1992</spage><epage>1995</epage><pages>1992-1995</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract>1 Departments of Ophthalmology and Visual Science and 2 Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska
Submitted 3 October 2005;
accepted in final form 15 December 2005
Horizontal cell (HC) to cone feedback helps establish the center-surround arrangement of visual receptive fields. It has been shown that HC activity influences cone synaptic output by altering the amplitude and voltage dependence of the calcium current ( I Ca ) in cones. In this study, we obtained voltage-clamp recordings simultaneously from cones and HCs to directly control the membrane potential of HCs and thereby measure the influence of HC membrane potential changes on I Ca in adjacent cones. Directly hyperpolarizing voltage clamped HCs produced a negative activation shift and increased the amplitude of I Ca in cones. Both of these effects were abolished by enhancing extracellular pH buffering capacity with HEPES. In contrast, addition of the gap junction blocker, carbenoxolone, did not significantly alter the shifts or amplitude changes in cone I Ca produced by changes in HC membrane potential. These results support the hypothesis that changes in the HC membrane potential alter the voltage dependence and amplitude of cone I Ca by altering extracellular pH levels at the synapse.
Address for reprint requests and other correspondence: W. B. Thoreson, Dept. of Ophthalmology, Univ. of Nebraska Medical Center, Durham Research Center, Omaha, NE 68198-5840 (E-mail: wbthores{at}unmc.edu )</abstract><cop>United States</cop><pub>Am Phys Soc</pub><pmid>16371457</pmid><doi>10.1152/jn.01042.2005</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Calcium - metabolism Calcium Signaling - physiology Cells, Cultured Feedback - physiology Hydrogen-Ion Concentration Membrane Potentials - physiology Patch-Clamp Techniques Retinal Cone Photoreceptor Cells - physiology Retinal Horizontal Cells - physiology Synaptic Transmission - physiology Urodela |
title | Feedback Effects of Horizontal Cell Membrane Potential on Cone Calcium Currents Studied With Simultaneous Recordings |
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