Loading…
Glucocorticoid Receptor Activation Lowers the Threshold for NMDA-Receptor-Dependent Homosynaptic Long-Term Depression in the Hippocampus Through Activation of Voltage-Dependent Calcium Channels
Christine M. Coussens 1 , 3 , D. Steven Kerr 2 , 3 , and Wickliffe C. Abraham 1 , 3 1 Department of Psychology, 2 Department of Pharmacology, and 3 Neuroscience Research Centre, University of Otago, Dunedin, New Zealand Coussens, Christine M., D. Steven Kerr, and Wickliffe C. Abraham. Glucocortic...
Saved in:
Published in: | Journal of neurophysiology 1997-07, Vol.78 (1), p.1-9 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Christine M. Coussens 1 , 3 ,
D. Steven Kerr 2 , 3 , and
Wickliffe C. Abraham 1 , 3
1 Department of Psychology, 2 Department of Pharmacology, and 3 Neuroscience Research Centre, University of Otago, Dunedin, New Zealand
Coussens, Christine M., D. Steven Kerr, and Wickliffe C. Abraham. Glucocorticoid receptor activation lowers the threshold for NMDA-receptor-dependent homosynaptic long-term depression in the hippocampus through activation of voltage-dependent calcium channels. J. Neurophysiol. 78: 1-9, 1997. The effects of the glucocorticoid receptor agonist RU-28362 on homosynaptic long-term depression (LTD) were examined in hippocampal slices obtained from adrenal-intact adult male rats. Field excitatory postsynaptic potentials were evoked by stimulation of the Schaffer collateral/commissural pathway and recorded in stratum radiatum of area CA1. Low-frequency stimulation (LFS) was delivered at LTD threshold (2 bouts of 600 pulses, 1 Hz, at baseline stimulation intensity). LFS of the Schaffer collaterals did not produce significant homosynaptic LTD in control slices. However, identical conditioning in the presence of the glucocorticoid receptor agonist RU-28362 (10 µM) produced a robust LTD, which was blocked by the selective glucocorticoid antagonist RU-38486. The LTD induced by glucocorticoid receptor activation was dependent on N -methyl- D -aspartate (NMDA) receptor activity, because the specific NMDA receptor antagonist D ( )-2-amino-5-phosphonopentanoic acid ( D -AP5) blocked the facilitation. However, the facilitation of LTD was not due to a potentiation of the isolated NMDA receptor potential by RU-28362. The facilitation of LTD byRU-28362 was also blocked by coincubation of the L-type voltage-dependent calcium channel (VDCC) antagonist nimodipine. Selective activation of the L-type VDCCs by the agonist Bay K 8644 also facilitated LTD induction. Both nimodipine and D -AP5 were effective in blocking the facilitation of LTD by Bay K 8644. These results indicate that L-type VDCCs can contribute to NMDAreceptor-dependent LTD induction. |
---|---|
ISSN: | 0022-3077 1522-1598 |
DOI: | 10.1152/jn.1997.78.1.1 |