Small mesenteric artery passive mechanical properties in adult APOE3 & APOE4 Mice

• Published in: Physiology (Bethesda, Md.) Md.), 2024-05, Vol.39 (S1)
• Main Authors: McMurray, Shelby, Walton, Isaiah, Gusek, Brikena, Stimpson, Anna, Jones, Carleton, Eckman, Delrae
• Format: Article
• Language: English
• ISSN: 1548-9213; 1548-9221
• DOI: 10.1152/physiol.2024.39.S1.2564

Abstract only Previous studies from our lab have demonstrated changes in vascular structure and function in posterior cerebral arteries and left common carotid artery (CA) in adult male and female mice expressing human-ApoE targeted replacement of APOE3 (B6.129P2-Apoe tm2(APOE*3)Mae N8) and APOE4 (B6.129P2-Apoe tm3(APOE*4)Mae N8) (Taconic Labs). Therefore, we hypothesized that similar changes may be observed in the systemic circulation. We isolated 4 th or 5th-order mesenteric arteries from adult 6-month-old male and female mice expressing hAPOE3 (n=5) or hAPOE4 (n=4). Mesenteric artery segments (5-8 mm in length) were isolated and cannulated on an arteriograph to assess vascular mechanical properties; lumen diameter, wall thickness, wall:lumen ratio, distensibility, stress versus strain (SvS), compliance, incremental modulus of elasticity (E inc ), and calculated pulse wave velocity under passive conditions (Ca 2+ -free Krebs plus diltiazem) at intraluminal pressures ranging from 10mmHg to 140mmHg. In addition, phenylephrine-induced contraction and potassium-induced contraction were assessed using Ca 2+ -containing Krebs. Data was analyzed using two-way ANOVA, Student’s t-test, and analysis of nonlinear fit. Values were considered statistically different at p