The Photocatalytic Inactivation Effect of Fe-Doped TiO 2 Nanocomposites on Leukemic HL60 Cells-Based Photodynamic Therapy

• Published in: International journal of photoenergy 2012-12, Vol.2012, p.1-8
• Main Authors: Huang, Kangqiang, Chen, Li, Liao, Meixiang, Xiong, Jianwen
• Format: Article
• Language: English
• ISSN: 1110-662X; 1687-529X
• DOI: 10.1155/2012/367072

The Fe-doped TiO 2 nanocomposites synthesized by a deposition-precipitation method were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), and UV-vis adsorption spectra and then were taken as a new “photosensitizer” for photodynamic therapy (PDT). The photocatalytic inactivation of Fe-doped TiO 2 on Leukemic HL60 cells was investigated using PDT reaction chamber based on LED light source, and the viability of HL60 cells was examined by Cell Counting Kit-8 (CCK-8) assay. The experimental results showed that the growth of leukemic HL60 cells was significantly inhibited by adding TiO 2 nanoparticles, and the inactivation efficiency could be effectively enhanced by the surface modification of TiO 2 nanoparticles with Fe doping. Furthermore, the optimized conditions were achieved at 5 wt% Fe/TiO 2 at a final concentration of 200  μ g/mL, in which up to 82.5% PDT efficiency for the HL60 cells can be obtained under the irradiation of 403 nm light (the power density is 5 mW/cm 2 ) within 60 minutes.