Biochemical Characterization, Action on Macrophages, and Superoxide Anion Production of Four Basic Phospholipases A 2 from Panamanian Bothrops asper Snake Venom

Bothrops asper (Squamata: Viperidae) is the most important venomous snake in Central America, being responsible for the majority of snakebite accidents. Four basic PLA 2 s (pMTX-I to -IV) were purified from crude venom by a single-step chromatography using a CM-Sepharose ion-exchange column (1.5 × 1...

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Bibliographic Details
Published in:BioMed research international 2013, Vol.2013, p.1-9
Main Authors: Rueda, Aristides Quintero, Rodríguez, Isela González, Arantes, Eliane C., Setúbal, Sulamita S., Calderon, Leonardo de A., Zuliani, Juliana P., Stábeli, Rodrigo G., Soares, Andreimar M.
Format: Article
Language:English
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Summary:Bothrops asper (Squamata: Viperidae) is the most important venomous snake in Central America, being responsible for the majority of snakebite accidents. Four basic PLA 2 s (pMTX-I to -IV) were purified from crude venom by a single-step chromatography using a CM-Sepharose ion-exchange column (1.5 × 15 cm). Analysis of the N-terminal sequence demonstrated that pMTX-I and III belong to the catalytically active Asp49 phospholipase A 2 subclass, whereas pMTX-II and IV belong to the enzymatically inactive Lys49 PLA 2 s-like subclass. The PLA 2 s isolated from Panama Bothrops asper venom (pMTX-I, II, III, and IV) are able to induce myotoxic activity, inflammatory reaction mainly leukocyte migration to the muscle, and induce J774A.1 macrophages activation to start phagocytic activity and superoxide production.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/789689