Genetic Polymorphism of msp 1 and msp 2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon

• Published in: Journal of parasitology research 2016, Vol.2016, p.1-7
• Main Authors: Yavo, William, Konaté, Abibatou, Mawili-Mboumba, Denise Patricia, Kassi, Fulgence Kondo, Tshibola Mbuyi, Marie L., Angora, Etienne Kpongbo, Menan, Eby I. Hervé, Bouyou-Akotet, Marielle K.
• Format: Article
• Language: English
• Subjects: alleles; Cote d'Ivoire; Gabon; genetic polymorphism; genetic variation; immune response; malaria; Plasmodium falciparum; polymerase chain reaction
• ISSN: 2090-0023; 2090-0031; 2090-0031
• DOI: 10.1155/2016/3074803

Introduction . The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods . Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d’Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp 1 and msp 2 genes. Results . The K1 allelic family was widespread in Côte d’Ivoire (64.6%) and in Gabon (56.6%). For msp 2, the 3D7 alleles were more prevalent (>70% in both countries) compared to FC27 alleles. In Côte d’Ivoire, the frequencies of multiple infections with msp 1 (45.1%) and msp 2 (40.3%) were higher than those found for isolates from Gabon, that is, 30.2% with msp 1 and 31.4% with msp 2. The overall complexity of infection was 1.66 (SD = 0.79) in Côte d’Ivoire and 1.58 (SD = 0.83) in Gabon. It decreased with age in Côte d’Ivoire in contrast to Gabon. Conclusion . Differences observed in some allelic families and in complexity profile may suggest an impact of epidemiological facies as well as immunological response on genetic variability of P. falciparum .