A 2A R Antagonists Upregulate Expression of GS and GLAST in Rat Hypoxia Model

• Published in: BioMed research international 2020, Vol.2020 (1), p.2054293
• Main Authors: Yu, Jun, Yan, Yan, Chen, Yiye, Zheng, Yan, Yu, Xiaoyan, Wang, Jialu, Wang, Yafu, Wang, Anken, Kang, Xiaoli, Cen, Jie, Dong, Lingyan, Li, Li, Zhao, Peiquan
• Format: Article
• Language: English
• Subjects: Adenosine A2 Receptor Antagonists - pharmacology; Amino Acid Transport System X-AG - genetics; Amino Acid Transport System X-AG - metabolism; Animals; Cell Survival - drug effects; Disease Models, Animal; Ependymoglial Cells - drug effects; Ependymoglial Cells - metabolism; Glutamate-Ammonia Ligase - genetics; Glutamate-Ammonia Ligase - metabolism; Hypoxia - enzymology; Hypoxia - genetics; Pyrazoles - pharmacology; Pyrazoles - toxicity; Pyrimidines - pharmacology; Pyrimidines - toxicity; Rats, Sprague-Dawley; Up-Regulation - drug effects
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2020/2054293

The aim of this study was to research the effects of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) in rat Müller cells and the effects of an adenosine A R antagonist (SCH 442416) on GS and GLAST in hypoxia both and . This study used RT-PCR and Western blotting to quantify the expressions of GS and GLAST under different hypoxic conditions as well as the expressions of GS and GLAST at different drug concentrations. A cell viability assay was used to assess drug toxicity. mRNA and protein expression of GS and GLAST in hypoxia Group 24 h was significantly increased. mRNA and protein expressions of GS and GLAST both increased in Group 1  M SCH 442416 compared with other groups. One micromolar SCH 442416 could upregulate GS and GLAST's activity in hypoxia both and . Hypoxia activates GS and GLAST in rat retinal Müller cells in a short time . (2) A R antagonists upregulate the activity of GS and GLAST in hypoxia both and .