Melatonin Supplementation to the Freezing Extender Improves the Quality of Canine Epididymal Spermatozoa

The present study aimed to determine the cryoprotective effect of melatonin supplements on the extender on the quality of canine epididymal spermatozoa. Epididymis from 20 castrated dogs were minced and incubated in Tris buffer. Spermatozoa were diluted in tris-citric acid-fructose (TCF) extender wi...

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Bibliographic Details
Published in:Andrologia 2023-10, Vol.2023, p.1-8
Main Authors: Fayez, Eman, Samir, Haney, El Sayed, Mohamed A. I., Rawash, Zaher M., Salama, Ali
Format: Article
Language:English
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Summary:The present study aimed to determine the cryoprotective effect of melatonin supplements on the extender on the quality of canine epididymal spermatozoa. Epididymis from 20 castrated dogs were minced and incubated in Tris buffer. Spermatozoa were diluted in tris-citric acid-fructose (TCF) extender with two doses of melatonin (0.002 and 0.0035 mol) as well as a control (0.0 mol). The samples were packed in 0.25 ml straws after 2 hr of equilibration at 5°C and kept in liquid nitrogen (−196°C). Sperm parameters such as viability, motility, integrity of acrosome, sperm membrane functioning, and DNA integrity were assessed after thawing (37°C for 30 s). In addition, lipid peroxidation was assessed using the determination of malondialdehyde (MDA) levels. Results revealed that adding 0.0035 mol melatonin to the cryopreservation medium enhanced (P
ISSN:0303-4569
1439-0272
DOI:10.1155/2023/7873399