Abstract 460: Influence of low oxygen conditions on the in vitro radiosensitizing effect of gemcitabine, in p53 wild type and p53 deficient non-small cell lung carcinoma cells

Introduction: It has been well documented that the efficacy of both chemotherapy and radiotherapy is directly linked with an adequate oxygen tension, and that hypoxic regions in solid tumors often contain viable cells that are intrinsically more resistant to anticancer treatment. As the antimetaboli...

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Published in:Cancer research (Chicago, Ill.) Ill.), 2010-04, Vol.70 (8_Supplement), p.460-460
Main Authors: Wouters, An, Pauwels, Bea, Lambrechts, Hilde AJ, Pattyn, Greet GO, Meijnders, Paul, Pauwels, Patrick, Peeters, Marc, Vermorken, Jan B., Lardon, Filip
Format: Article
Language:English
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Summary:Introduction: It has been well documented that the efficacy of both chemotherapy and radiotherapy is directly linked with an adequate oxygen tension, and that hypoxic regions in solid tumors often contain viable cells that are intrinsically more resistant to anticancer treatment. As the antimetabolite gemcitabine is a potent radiosensitizer, often used in the clinic, the influence of oxygen deficiency on the radiosensitizing effect of gemcitabine was investigated. Materials & methods: The human tumor cell lines included were A549 (lung carcinoma cell line, wt p53), A549-E6 (transduced with the HPV type 16 E6 gene, abrogating the p53 function) and A549-LXSN (vector transfected control, functional p53). Anoxic conditions (
ISSN:0008-5472
1538-7445
DOI:10.1158/1538-7445.AM10-460