Abstract 3066: Direct detection and enumeration of circulating tumor cells (CTCs) in blood by CastRT-qPCR

Statistical data suggest that only approximately 25% of cancer patients will respond to the same treatment. Enumeration and molecular characterization of circulating tumor cells (CTCs) promise to be valuable for cancer cell diagnosis, survival prognosis, and treatment guidance. However, CTCs are ver...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2011-04, Vol.71 (8_Supplement), p.3066-3066
Main Authors: Deng, David X., Sprout, Scott, Bao, Yun, Merrill, David, Brzoska, Pius, Wang, Yu, Chen, Caifu
Format: Article
Language:English
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Summary:Statistical data suggest that only approximately 25% of cancer patients will respond to the same treatment. Enumeration and molecular characterization of circulating tumor cells (CTCs) promise to be valuable for cancer cell diagnosis, survival prognosis, and treatment guidance. However, CTCs are very rare. There is no sensitive and specific assay available for analyzing CTCs from whole blood without extensive enrichment processes. Sample enrichment causes target cells loss, expensive and time-consuming. Here we report a highly specific competitive allele-specific TaqMan-based RT-qPCR (castRT-qPCR) for rare copies of DNA mutation detection. CastPCR can detect rare copies of mutant alleles for up to one in 10 million with a wide dynamic range of more than 6-log orders and
ISSN:0008-5472
1538-7445
DOI:10.1158/1538-7445.AM2011-3066